The Effect of Titanium Implants with Micro-arc Oxidation Coatings on Osseointegration in Rabbits

• Main Authors: Cheng-ChungHsu, 徐政忠
• Other Authors: Tzer-Min Lee
• Format: Others
• Language: en_US
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/91387333121763168447

碩士 === 國立成功大學 === 口腔醫學研究所 === 101 === Today, the use of dental implants has become a common treatment procedure in the replacement of missing teeth. The key of the success of dental implant is the establishment of osseointegration between implant surface and surrounding alveolar bone. The design, chemical composition and topography of the implant surface can influence the extent of osseointegration. Many literatures show that SLA(Sandblasted, Large grit, Acid-etched) surface improved osseointegration with a high percentage of bone-implant contact and high removal torque values. Strontium (Sr), which has the ability to inhibit bone resorption and stimulate bone formation, is beneficial for biological application for bone regeneration. The micro-arc oxidation (MAO) treatment of titanium (Ti) have a beneficial effect on the biocompatibility of the Ti implant. We aim to evaluate the response of implants with strontium coating by micro-arc oxidation (MAO-Sr) and compare the result of SLA surface in tibia of rabbits. To prepare the animal study, 9 New Zealand white rabbits will be used. Three implants will be inserted into the each tibia of rabbits: MAO, MAOSr and SLA surface. At 2 weeks after implant insertion, 3 rabbits will be sacrificed. The tissue around implant will be removed for real-time polymerase chain reaction (RT-PCR). At 4 weeks after implant insertion, 6 rabbits will be sacrificed. The tibia will be removed for histomorphmetric analysis and removal torque assay. A bone-to-implant contact (BIC), functionally remove torque test and genes related to bone formation and resorption will be examined. There was no significant differences in the removal torque values between SLA, MAO and MAOSr microimplants. However, the MAOSr microimplants had statistically significant higher bone-to-implant contact (BIC) than the SLA and MAO microimplants. There was no significant differences between the SLA and the MAO group. In RT-PCR analysis, increasing mRNA expression of osteogenesis-related genes (BSP, OCN, CD44 and CD73) was noted in the MAOSr group. In addition, down-regulating mRNA expression of the osteoclastogenesis-related genes (RANKL and RANK) and up-regulating the OPG expression was also noted in the MAOSr group. The results indicate that the local delivery of strontium from the MAOSr surfaces can not only promote osteogenesis but also inhibit osteoclastogenesis by up-regulating the osteogenesis-related genes and down-regulating the osteoclastogenesis-related genes. The MAOSr surface can promote early osseointegration of the titanium microimplants comparing to the SLA and MAO surface.