| Summary: | 碩士 === 國立成功大學 === 工程科學系碩博士班 === 101 === This study, a novel micro-electrode biochip for immunoassay was applied to detect sandwich-immunoassay of carcinoembryonic, a gold nanoparticles (AuNPs) as a label of antibody to enhance immunoassay impedance quantitative analysis detection. Compared with traditional enzyme-linked immunosorbent assay (ELISA), we utilized the LCR meter instrument to detect the electric signal on immune-impedance chip. By detecting the impedance changes because of sandwich-immunoassay reaction, a sandwich-immunoassay detection system was established. In this study, the experiment was divided into micro-electrode chip and microchannel electrode chip. Firstly, in micro-electrode chip, we used surface chemical modification method to modify the surface of immune-impedance chip. Then using the contact angle measurement instrument and the fluorescent labeled antibody method verify the results of chip modification. Only used 25 μL of samples that immune detection can be work in the immune detection system, the total time of the immunoassay was about 10 minutes, and the minimum signal detection was 1 ng/mL of carcinoembryonic. Secondly, we combined micro-electrode chip with microchannel into microchannel electrode chip and not by using surface chemical modification. It only used 10 μL of samples in the immune detection system. The total detection time of the immunoassay was about 8 minutes, and the minimum signal detection of microchannel electrode chip was 0.1 ng/mL of carcinoembryonic. Additionally, micro-electrode chip and microchannel electrode chip have the best distinction under 100 Hz in this detection system, according to the relationship between the impedance and carcinoembryonic concentration. In this study, combined impedance changing detection and nanogold labeling technique in sandwich-immunoassay was a novel notion. Impedance measurement was performed for quantitative and qualitative analysis in the aim of decreasing detection time and lowering assay cost. It provided a new immunoassay method for drug-detection and medical analysis sciene.
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