| Summary: | 碩士 === 國立成功大學 === 工程科學系碩博士班 === 101 === This study successfully develops the macromolecule membrane filtration chip to separate blood cells and plasma, and develops system which combine micropump with filtration chip. The filtration chip is based on dead-end filtration to separate blood cells in whole blood. The dead-end filtration used pressure as force to make the fluidic pass through the membrane and get the filtrate. The filtration chip uses the laser ablation of MEMS process to manufacture polymethylmethacrylate (PMMA) filtration chip. Then, combined macromolecule membrane with PMMA chip to complete whole blood filtration chip. This study was discussed the plasma collection under different membrane sizes(56.25 mm2, 100 mm2 and 225 mm2), different volume of whole blood(60 μL, 80 μL, 100 μL, 120 μL, 140 μL, 160 μL and 180 μL) and different frequencies(10 Hz, 20 Hz, 30 Hz, 40 Hz, 50 Hz and 60 Hz). When the membrane size is 225 mm2, volume of whole blood is 140 μL and frequencies are between 40 Hz and 60 Hz, the plasma collection rate is up to 58%. The frequency between 10 Hz and 50 Hz have small hemolysis(hemolysis rate is between 0.33% and 0.38%). Finally, this study develops the rapid separation system which combined time-controlled micropump and disposable filtration chip was made by laser ablation and double-side material, the chip size is 30 mm× 20 mm× 5 mm. This study has better effect on blood cells and plasma separation than other experiment results, and it can rapidly finish separation in 10 minutes, and avoid hemolysis.
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