Redox- and pH-responsive complex micelles assembled from saccharide- and lipoic acid-modified poly(L-lysine) and poly(acrylic acid)
碩士 === 國立成功大學 === 化學工程學系碩博士班 === 101 === In this study, novel pH and redox-responsive, cellular targeting polyelectrolyte complex (PEC) particles were developed. By mixing the polycation and polyanion, lactobionolatone modified poly(L-lysine)-graft-lipoic acid (PLL-g-Lipo-g-Lac) and poly acrylic aci...
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ndltd-TW-101NCKU50630642016-03-18T04:42:17Z http://ndltd.ncl.edu.tw/handle/29332415563334527584 Redox- and pH-responsive complex micelles assembled from saccharide- and lipoic acid-modified poly(L-lysine) and poly(acrylic acid) 醣與硫辛酸修飾聚賴胺酸與聚丙烯酸自組裝之還原及酸鹼應答複合高分子 Ping-LunHsieh 謝秉倫 碩士 國立成功大學 化學工程學系碩博士班 101 In this study, novel pH and redox-responsive, cellular targeting polyelectrolyte complex (PEC) particles were developed. By mixing the polycation and polyanion, lactobionolatone modified poly(L-lysine)-graft-lipoic acid (PLL-g-Lipo-g-Lac) and poly acrylic acid (PAA), PEC particles can be formed with excess polyelectrolyte and saccharide group on the corona. PLL polypeptides were prepared by synthesizing poly(Z-L-lysine) (PZLL) via ring-opening polymerization (ROP) from Z-L-lysine N-carboxyanhydrides (NCAs) and subsequently removing the protecting Z group. PLL-g-Lipo-g-Lac graft copolypeptides with different polypeptide chain lengths and grafting degrees of lipoic acid were synthesized. The grafting degrees of lipoic acid and saccharide group were determined by Nuclear Magnetic Resonance Spectrophotometer (NMR). The size, charge, chain conformation, and morphology of the as-prepared PEC particles at different solution conditions were characterized by dynamic light scattering (DLS), aqueous electrophoresis, transmission electron microscopy (TEM) and circular dichroism (CD) measurements. The positively and negatively charged PEC particles with size ranged between 50 and 200 nm can be prepared by varying polymer chain length, grafting degree, and composition. The experimental data revealed that these PEC particles can response to the pH and oxidation/reduction changes in the environment. The selective lectin binding experiments confirmed that the galactose units on the particles can be used in biorecognition applications. Combining their dual-response and liver cell targeting ability, these PEC particles could be useful in biomedical fields such as drug delivery. Jeng-Shiung Jan 詹正雄 2013 學位論文 ; thesis 81 zh-TW |
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碩士 === 國立成功大學 === 化學工程學系碩博士班 === 101 === In this study, novel pH and redox-responsive, cellular targeting polyelectrolyte complex (PEC) particles were developed. By mixing the polycation and polyanion, lactobionolatone modified poly(L-lysine)-graft-lipoic acid (PLL-g-Lipo-g-Lac) and poly acrylic acid (PAA), PEC particles can be formed with excess polyelectrolyte and saccharide group on the corona. PLL polypeptides were prepared by synthesizing poly(Z-L-lysine) (PZLL) via ring-opening polymerization (ROP) from Z-L-lysine N-carboxyanhydrides (NCAs) and subsequently removing the protecting Z group. PLL-g-Lipo-g-Lac graft copolypeptides with different polypeptide chain lengths and grafting degrees of lipoic acid were synthesized. The grafting degrees of lipoic acid and saccharide group were determined by Nuclear Magnetic Resonance Spectrophotometer (NMR). The size, charge, chain conformation, and morphology of the as-prepared PEC particles at different solution conditions were characterized by dynamic light scattering (DLS), aqueous electrophoresis, transmission electron microscopy (TEM) and circular dichroism (CD) measurements. The positively and negatively charged PEC particles with size ranged between 50 and 200 nm can be prepared by varying polymer chain length, grafting degree, and composition. The experimental data revealed that these PEC particles can response to the pH and oxidation/reduction changes in the environment. The selective lectin binding experiments confirmed that the galactose units on the particles can be used in biorecognition applications. Combining their dual-response and liver cell targeting ability, these PEC particles could be useful in biomedical fields such as drug delivery.
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author2 |
Jeng-Shiung Jan |
author_facet |
Jeng-Shiung Jan Ping-LunHsieh 謝秉倫 |
author |
Ping-LunHsieh 謝秉倫 |
spellingShingle |
Ping-LunHsieh 謝秉倫 Redox- and pH-responsive complex micelles assembled from saccharide- and lipoic acid-modified poly(L-lysine) and poly(acrylic acid) |
author_sort |
Ping-LunHsieh |
title |
Redox- and pH-responsive complex micelles assembled from saccharide- and lipoic acid-modified poly(L-lysine) and poly(acrylic acid) |
title_short |
Redox- and pH-responsive complex micelles assembled from saccharide- and lipoic acid-modified poly(L-lysine) and poly(acrylic acid) |
title_full |
Redox- and pH-responsive complex micelles assembled from saccharide- and lipoic acid-modified poly(L-lysine) and poly(acrylic acid) |
title_fullStr |
Redox- and pH-responsive complex micelles assembled from saccharide- and lipoic acid-modified poly(L-lysine) and poly(acrylic acid) |
title_full_unstemmed |
Redox- and pH-responsive complex micelles assembled from saccharide- and lipoic acid-modified poly(L-lysine) and poly(acrylic acid) |
title_sort |
redox- and ph-responsive complex micelles assembled from saccharide- and lipoic acid-modified poly(l-lysine) and poly(acrylic acid) |
publishDate |
2013 |
url |
http://ndltd.ncl.edu.tw/handle/29332415563334527584 |
work_keys_str_mv |
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