Redox- and pH-responsive complex micelles assembled from saccharide- and lipoic acid-modified poly(L-lysine) and poly(acrylic acid)

碩士 === 國立成功大學 === 化學工程學系碩博士班 === 101 === In this study, novel pH and redox-responsive, cellular targeting polyelectrolyte complex (PEC) particles were developed. By mixing the polycation and polyanion, lactobionolatone modified poly(L-lysine)-graft-lipoic acid (PLL-g-Lipo-g-Lac) and poly acrylic aci...

Full description

Bibliographic Details
Main Authors: Ping-LunHsieh, 謝秉倫
Other Authors: Jeng-Shiung Jan
Format: Others
Language:zh-TW
Published: 2013
Online Access:http://ndltd.ncl.edu.tw/handle/29332415563334527584
id ndltd-TW-101NCKU5063064
record_format oai_dc
spelling ndltd-TW-101NCKU50630642016-03-18T04:42:17Z http://ndltd.ncl.edu.tw/handle/29332415563334527584 Redox- and pH-responsive complex micelles assembled from saccharide- and lipoic acid-modified poly(L-lysine) and poly(acrylic acid) 醣與硫辛酸修飾聚賴胺酸與聚丙烯酸自組裝之還原及酸鹼應答複合高分子 Ping-LunHsieh 謝秉倫 碩士 國立成功大學 化學工程學系碩博士班 101 In this study, novel pH and redox-responsive, cellular targeting polyelectrolyte complex (PEC) particles were developed. By mixing the polycation and polyanion, lactobionolatone modified poly(L-lysine)-graft-lipoic acid (PLL-g-Lipo-g-Lac) and poly acrylic acid (PAA), PEC particles can be formed with excess polyelectrolyte and saccharide group on the corona. PLL polypeptides were prepared by synthesizing poly(Z-L-lysine) (PZLL) via ring-opening polymerization (ROP) from Z-L-lysine N-carboxyanhydrides (NCAs) and subsequently removing the protecting Z group. PLL-g-Lipo-g-Lac graft copolypeptides with different polypeptide chain lengths and grafting degrees of lipoic acid were synthesized. The grafting degrees of lipoic acid and saccharide group were determined by Nuclear Magnetic Resonance Spectrophotometer (NMR). The size, charge, chain conformation, and morphology of the as-prepared PEC particles at different solution conditions were characterized by dynamic light scattering (DLS), aqueous electrophoresis, transmission electron microscopy (TEM) and circular dichroism (CD) measurements. The positively and negatively charged PEC particles with size ranged between 50 and 200 nm can be prepared by varying polymer chain length, grafting degree, and composition. The experimental data revealed that these PEC particles can response to the pH and oxidation/reduction changes in the environment. The selective lectin binding experiments confirmed that the galactose units on the particles can be used in biorecognition applications. Combining their dual-response and liver cell targeting ability, these PEC particles could be useful in biomedical fields such as drug delivery. Jeng-Shiung Jan 詹正雄 2013 學位論文 ; thesis 81 zh-TW
collection NDLTD
language zh-TW
format Others
sources NDLTD
description 碩士 === 國立成功大學 === 化學工程學系碩博士班 === 101 === In this study, novel pH and redox-responsive, cellular targeting polyelectrolyte complex (PEC) particles were developed. By mixing the polycation and polyanion, lactobionolatone modified poly(L-lysine)-graft-lipoic acid (PLL-g-Lipo-g-Lac) and poly acrylic acid (PAA), PEC particles can be formed with excess polyelectrolyte and saccharide group on the corona. PLL polypeptides were prepared by synthesizing poly(Z-L-lysine) (PZLL) via ring-opening polymerization (ROP) from Z-L-lysine N-carboxyanhydrides (NCAs) and subsequently removing the protecting Z group. PLL-g-Lipo-g-Lac graft copolypeptides with different polypeptide chain lengths and grafting degrees of lipoic acid were synthesized. The grafting degrees of lipoic acid and saccharide group were determined by Nuclear Magnetic Resonance Spectrophotometer (NMR). The size, charge, chain conformation, and morphology of the as-prepared PEC particles at different solution conditions were characterized by dynamic light scattering (DLS), aqueous electrophoresis, transmission electron microscopy (TEM) and circular dichroism (CD) measurements. The positively and negatively charged PEC particles with size ranged between 50 and 200 nm can be prepared by varying polymer chain length, grafting degree, and composition. The experimental data revealed that these PEC particles can response to the pH and oxidation/reduction changes in the environment. The selective lectin binding experiments confirmed that the galactose units on the particles can be used in biorecognition applications. Combining their dual-response and liver cell targeting ability, these PEC particles could be useful in biomedical fields such as drug delivery.
author2 Jeng-Shiung Jan
author_facet Jeng-Shiung Jan
Ping-LunHsieh
謝秉倫
author Ping-LunHsieh
謝秉倫
spellingShingle Ping-LunHsieh
謝秉倫
Redox- and pH-responsive complex micelles assembled from saccharide- and lipoic acid-modified poly(L-lysine) and poly(acrylic acid)
author_sort Ping-LunHsieh
title Redox- and pH-responsive complex micelles assembled from saccharide- and lipoic acid-modified poly(L-lysine) and poly(acrylic acid)
title_short Redox- and pH-responsive complex micelles assembled from saccharide- and lipoic acid-modified poly(L-lysine) and poly(acrylic acid)
title_full Redox- and pH-responsive complex micelles assembled from saccharide- and lipoic acid-modified poly(L-lysine) and poly(acrylic acid)
title_fullStr Redox- and pH-responsive complex micelles assembled from saccharide- and lipoic acid-modified poly(L-lysine) and poly(acrylic acid)
title_full_unstemmed Redox- and pH-responsive complex micelles assembled from saccharide- and lipoic acid-modified poly(L-lysine) and poly(acrylic acid)
title_sort redox- and ph-responsive complex micelles assembled from saccharide- and lipoic acid-modified poly(l-lysine) and poly(acrylic acid)
publishDate 2013
url http://ndltd.ncl.edu.tw/handle/29332415563334527584
work_keys_str_mv AT pinglunhsieh redoxandphresponsivecomplexmicellesassembledfromsaccharideandlipoicacidmodifiedpolyllysineandpolyacrylicacid
AT xièbǐnglún redoxandphresponsivecomplexmicellesassembledfromsaccharideandlipoicacidmodifiedpolyllysineandpolyacrylicacid
AT pinglunhsieh tángyǔliúxīnsuānxiūshìjùlàiànsuānyǔjùbǐngxīsuānzìzǔzhuāngzhīháiyuánjísuānjiǎnyīngdáfùhégāofēnzi
AT xièbǐnglún tángyǔliúxīnsuānxiūshìjùlàiànsuānyǔjùbǐngxīsuānzìzǔzhuāngzhīháiyuánjísuānjiǎnyīngdáfùhégāofēnzi
_version_ 1718207976671543296