| Summary: | 碩士 === 國立成功大學 === 化學工程學系碩博士班 === 101 === The aim of this study is to prepare several scaffolds containing hydrophilic/hydrophobic bilayer surface and investigate the physical properties and biocompatibility of the scaffold as prepared. By using electrospinning and film formation technique, the nanofibers and thin films made by hydrophilic polyvinyl alcohol (PVA) and hydrophobic polystyrene (PS) were prepared. A variety of bilayer scaffolds was then fabricated by combining these hydrophilic/hydrophobic nanofiber and thin film structure. By changing the morphology of these hydrophilic and hydrophobic combinations, the surface characteristics as well as cell attachment and growth behavior on these bilayer scaffolds were analyzed.
Scanning electron microscopy (SEM) was utilized to examine the surface morphology of bilayer scaffold in ambient environment as well as being immersed in water. From the results of viscometer and dynamic mechanical analyzer (DMA), the crosslinking reaction of PVA by glutaraldehyde (GA) would affect the viscosity of solution, fibrous morphology and mechanical strength of scaffolds. The correlations between the preparation scheme and roughness and wettability of bilayer scaffold as resulted was determined by atomic force microscopy (AFM), water contact angle and water uptake.
For biocompatibility evaluation, in vitro cell culture using murine NIH-3T3 fibroblasts was utilized. MTT assay was used to assess the cell viability and proliferation. Cell morphology was determined by SEM for those attached on the bilayer scaffold for 1 and 7 days. It was clearly noted that the cell morphology and cell proliferation were affected by the surface characteristics of the bilayer scaffold. By increasing the surface roughness and hydrophilicity, the cell attachment and proliferation on the bilayer scaffold is also increased. It was concluded the bilayer scaffold formed by the PS nanofibers on the bottom and PVA nanofibers on the top was the most biocompatible one in this investigation.
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