| Summary: | 碩士 === 國立成功大學 === 生物科技研究所碩博士班 === 101 === Giant grouper is an economically important fish species in Taiwan’s aquaculture industry. Thus, we are attempting to develop a technique that can investigate the parent-offspring/sibling relationship of giant grouper. This technique will improve the inbreeding management of fish farms and minimizes the chance of close relative mating to as lowest as possible. In this study, we would like to apply two systems which are microsatellite and mitochondrial genetic markers as tools to analyze genetic distance of giant grouper population. The high variable mitochondrial D-loop sequence is selected to study the siblings’ relationship of giant groupers. Analysis of data collected from 3 fish farms, with total samples of 118 giant groupers, we found and categorized 42 haplotypes as well as calculate their genetic distance. Genetically close relative giant groupers will be separated to different farms. Moreover, in this project, microsatellites system was applied as parent-offspring investigation tool. 6 different microsatellite loci were used as marker, which are obeying Hardy-Weinberg equilibrium and do not exhibit genetic linkage within each other. Therefore, with high number of possible combinations could avoid chance of misinterpretation and can be practically in aquaculture industry. Combining both techniques would enhance the effectiveness in Taiwan’s giant grouper inbreeding management.
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