AC Electrokinetics-based Ripple Structure Fabrication and Its Application on Bioparticle Manipulation

• Main Authors: Sheng-ChuanChiang, 蔣盛全
• Other Authors: Hsien-Chang Chang
• Format: Others
• Language: en_US
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/75440449730676284313

碩士 === 國立成功大學 === 生物醫學工程學系 === 101 === In recent years, a lot of research group were conducting work on isolation and detection of biological sample. There were many developing method with variety of different technologies for the particular biological particles in the separation stage. In this study, we proposed the combination of the micro-ripple structure generated by non-uniform electric field and particle manipulation by electrodynamics to achieve rapid detection, high sensitivity and without biological calibration biochips. The micro-ripple structure was made of ultraviolet-curable adhesive that used the UV oven to cure. The structure combined with the DEP and ACEO drag force which have different from the usual dielectrokinetics. In this study, the balance of DEP and ACEO drag force at the crest of micro-ripple structures, so that the particles can produce mixing results. The phoneme can be applied by the combine effects strengthen of the modified antigen-antibody on particles and investigated the combination coefficients. Staphylococcus aureus and Anti-protein A IgG used in a mixing chip study, the agglutination reactions generated based on the bonding strength. Under the frequency control, we can observe difference with and without agglutination of Staphylococcus aureus. This study provides a new platform by mixing particles of different particle surface modification can make a specific capture and detection of bacteria.