Study on a Ring-shaped Interdigitated Electrode Controlled by AC Electrokinetic for Concentrating Bio-particles

• Main Authors: Jyun-HongWang, 王竣弘
• Other Authors: Hsien-Chang Chang
• Format: Others
• Language: en_US
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/08732930865932931514

碩士 === 國立成功大學 === 生物醫學工程學系 === 101 === In general, there are some problems of detection in hospital such as time consuming and complicated detection. In this research, a micro-concentrator platform for medical combined micro electro mechanical systems (MEMS) and electrokinetic is designed, then the sample in solution can be concentrated in few minutes. In this study, an electrokinetic-based ring interdigitated electrode platform was designed. Combined dielectrophoresis (DEP) generated by non-uniform electric field and AC electroosmotic flow (ACEO) to manipulate particles in the solution to transmit and concentrated, and applied to biological particles such as bacteria immediately concentrated. When the bacteria solution (~100 L) was dropped to micro-concentrator platform and AC signal (120 Vpp, 1 kHz) is applied to the device, the bacteria in the solution were transmitted and concentrated by AC electroosmotic flow (ACEO) and dielectrophoresis (DEP) without external micro-syringe. In this study, Vibrio, Escherichia coli and Staphylococcus aureu were used for transmission and concentration by interaction of ACEO and DEP applied appropriate AC signal and frequency. In ten minutes, the different concentrations of bacteria (106 ~ 104 CFU/mL) could be distinguished. In the future, the platform may be applied to the low concentration detection, such as rapid test on urinary tract infection, etc.