| Summary: | 博士 === 國立成功大學 === 資訊工程學系碩博士班 === 101 === In molecular biology, the study of gene expression plays an important role because the amount of gene expression reflects the amount of functional proteins, and there are many factors that can affect gene expression. In epigenetics, the research focuses on the factors rather than just changes in the underlying nucleotide sequence, and refers to relevant modifications to the genome that do not involve a change in DNA sequence. Gene expression can be controlled through epigenetic regulation such as chromatin remodeling or histone modification.
In this dissertation, multiple genome-wide profiles of epigenetic target in Saccharomyces cerevisiae were used for analysis on the chromatin regulation. For profiles of nucleosome positioning, we quantified the nucleosome stability by incorporating mass sequencing data, and provided a significant flanking pattern of sequence preference around nucleosomal DNA for a stable nucleosome. For profiles of histone-related proteins, the gene expression coupling with binding sites provide a framework to study the function of the histone variant in response to oleate, and to study the function of histone chaperone on the gene silencing at telomere-proximal regions.
Our analysis on epigenetics revealed the function of the histone-related proteins, and also provided a reliable measure for nucleosome stability. Both studies contribute to the field of epigenetic regulation, and relevant results can extend to further researches regarding the mechanism of chromatin remodeling.
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