Epigenetic Analysis for Genome-wide Profiles of Saccharomyces cerevisiae

博士 === 國立成功大學 === 資訊工程學系碩博士班 === 101 === In molecular biology, the study of gene expression plays an important role because the amount of gene expression reflects the amount of functional proteins, and there are many factors that can affect gene expression. In epigenetics, the research focuses on th...

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Main Authors: Chan-HsienLin, 林展賢
Other Authors: Jung-Hsien Chiang
Format: Others
Language:en_US
Published: 2013
Online Access:http://ndltd.ncl.edu.tw/handle/83274725522843475811
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spelling ndltd-TW-101NCKU53920452015-10-13T22:51:43Z http://ndltd.ncl.edu.tw/handle/83274725522843475811 Epigenetic Analysis for Genome-wide Profiles of Saccharomyces cerevisiae 基於酵母菌全基因組分析之表觀遺傳學研究 Chan-HsienLin 林展賢 博士 國立成功大學 資訊工程學系碩博士班 101 In molecular biology, the study of gene expression plays an important role because the amount of gene expression reflects the amount of functional proteins, and there are many factors that can affect gene expression. In epigenetics, the research focuses on the factors rather than just changes in the underlying nucleotide sequence, and refers to relevant modifications to the genome that do not involve a change in DNA sequence. Gene expression can be controlled through epigenetic regulation such as chromatin remodeling or histone modification. In this dissertation, multiple genome-wide profiles of epigenetic target in Saccharomyces cerevisiae were used for analysis on the chromatin regulation. For profiles of nucleosome positioning, we quantified the nucleosome stability by incorporating mass sequencing data, and provided a significant flanking pattern of sequence preference around nucleosomal DNA for a stable nucleosome. For profiles of histone-related proteins, the gene expression coupling with binding sites provide a framework to study the function of the histone variant in response to oleate, and to study the function of histone chaperone on the gene silencing at telomere-proximal regions. Our analysis on epigenetics revealed the function of the histone-related proteins, and also provided a reliable measure for nucleosome stability. Both studies contribute to the field of epigenetic regulation, and relevant results can extend to further researches regarding the mechanism of chromatin remodeling. Jung-Hsien Chiang 蔣榮先 2013 學位論文 ; thesis 90 en_US
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description 博士 === 國立成功大學 === 資訊工程學系碩博士班 === 101 === In molecular biology, the study of gene expression plays an important role because the amount of gene expression reflects the amount of functional proteins, and there are many factors that can affect gene expression. In epigenetics, the research focuses on the factors rather than just changes in the underlying nucleotide sequence, and refers to relevant modifications to the genome that do not involve a change in DNA sequence. Gene expression can be controlled through epigenetic regulation such as chromatin remodeling or histone modification. In this dissertation, multiple genome-wide profiles of epigenetic target in Saccharomyces cerevisiae were used for analysis on the chromatin regulation. For profiles of nucleosome positioning, we quantified the nucleosome stability by incorporating mass sequencing data, and provided a significant flanking pattern of sequence preference around nucleosomal DNA for a stable nucleosome. For profiles of histone-related proteins, the gene expression coupling with binding sites provide a framework to study the function of the histone variant in response to oleate, and to study the function of histone chaperone on the gene silencing at telomere-proximal regions. Our analysis on epigenetics revealed the function of the histone-related proteins, and also provided a reliable measure for nucleosome stability. Both studies contribute to the field of epigenetic regulation, and relevant results can extend to further researches regarding the mechanism of chromatin remodeling.
author2 Jung-Hsien Chiang
author_facet Jung-Hsien Chiang
Chan-HsienLin
林展賢
author Chan-HsienLin
林展賢
spellingShingle Chan-HsienLin
林展賢
Epigenetic Analysis for Genome-wide Profiles of Saccharomyces cerevisiae
author_sort Chan-HsienLin
title Epigenetic Analysis for Genome-wide Profiles of Saccharomyces cerevisiae
title_short Epigenetic Analysis for Genome-wide Profiles of Saccharomyces cerevisiae
title_full Epigenetic Analysis for Genome-wide Profiles of Saccharomyces cerevisiae
title_fullStr Epigenetic Analysis for Genome-wide Profiles of Saccharomyces cerevisiae
title_full_unstemmed Epigenetic Analysis for Genome-wide Profiles of Saccharomyces cerevisiae
title_sort epigenetic analysis for genome-wide profiles of saccharomyces cerevisiae
publishDate 2013
url http://ndltd.ncl.edu.tw/handle/83274725522843475811
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