Inhibition of Migration and Invasion of Hepatocellular Carcinoma Huh7 Cells by Lauryl Gallate

• Main Authors: Lin, Weichieh, 林威傑
• Other Authors: Wu, Lichen
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/93364938549837135535

碩士 === 國立暨南國際大學 === 應用化學系 === 101 === Hepatocellular carcinoma is one of the most common types of malignancies with poor prognosis. It is the third leading cause of cancer-related death worldwide. In Taiwan, hepatocellular carcinoma is the leading cause of cancer death, with approximately 8000 new cases diagnosed and 7000 deaths occurring annually. Although substantial progress has been made in developing chemotherapeutic treatments for hepatocellular carcinoma, drug efficacy is often outweighed by undesirable side effects. Therefore, new drugs with a higher therapeutic index are urgently needed for hepatocellular carcinoma patients. Gallic acid and its derivatives, commonly used in cosmetics, pharmaceutical, and food industries as antioxidants, have been reported with antiviral, anti-inflammatory, and antitumor properties. In this study, the effects and the molecular mechanisms of gallic acid (3,4,5-trihydroxybenzoic acid, GA) and its esters, octyl gallate (3,4,5-trihydroxybenzoic acid octyl ester, E-311, OG), and lauryl gallate (Dodecyl 3,4,5-trihydroxybenzoate, E-312, LG) on the growth, survival and invasion were examined in human hepatocellular carcinoma cell lines. Our results showed that treatment of Huh7 cells with GA, OG and LG caused a significantly growth inhibition, with IC50 value of 235, 25 and 10 μM respectively. Under IC90 concentration treatment, GA markedly stimulated ROS production. In contrast, OG and LG exhibited antioxidative effect. Moreover, all the tested compounds prevented Huh7 cell migration at sub-lethal dose treatment. We found LG not only decreased MMP-2 and MMP-9 activity but also blocked FAK/Paxillin pathway to suppress cell migrated and invasive ability.