A new gadolinium(III) complex with dansyl based copper MRI sensor
碩士 === 國立交通大學 === 分子醫學與生物工程研究所 === 101 === In the present study, we designed and synthesized MR-optical imaging agent, [Gd(DNS-DOTA)], for the detection of the divalent copper ion(Cu2+). Luminescence lifetime measurement shows the number of inner-sphere water molecules (q) in [Gd(DNS-DOTA)] is zero....
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ndltd-TW-101NCTU55380062015-10-13T22:45:37Z http://ndltd.ncl.edu.tw/handle/94728462100636621483 A new gadolinium(III) complex with dansyl based copper MRI sensor 具有Dansyl結構的銅離子磁振造影對比劑之合成與特性研究 Lin, Hsin-Ping 林欣平 碩士 國立交通大學 分子醫學與生物工程研究所 101 In the present study, we designed and synthesized MR-optical imaging agent, [Gd(DNS-DOTA)], for the detection of the divalent copper ion(Cu2+). Luminescence lifetime measurement shows the number of inner-sphere water molecules (q) in [Gd(DNS-DOTA)] is zero. However, upon addition of Cu2+ to [Gd(DNS-DOTA)], the q value changes from 0 to 1. Consequently increase in the relaxivity of [Gd(DNS-DOTA)] was observed in the presence of Cu2+. Consistent to relaxometric study, magnetic resonance imaging (MRI) experiments display the increase in signal strength with the increase in the Cu2+ ion concentration. In addition, attenuation in the fluorescence intensity of [Gd(DNS-DOTA)] was observed on adding Cu2+ ion which substantiate that the coordination profile of [Gd(DNS-DOTA)] is alter in the presence of Cu2+ ion. High coordination preference of [Gd(DNS-DOTA)] for Cu2+ ion over other metal ion was observed under the same condition. Last but not least, confocal imaging experiment shows that the fluorescence intensity [Gd(DNS-DOTA)] suppressed on incubating with 3T3 cell pretreated with Cu2+. Therefore, [Gd(DNS-DOTA)] is a potential MR-optical imaging agent for the detection of intracellular Cu2+ ion with high specificity. Wang, Yun-Ming 王雲銘 2012 學位論文 ; thesis 58 zh-TW |
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碩士 === 國立交通大學 === 分子醫學與生物工程研究所 === 101 === In the present study, we designed and synthesized MR-optical imaging agent, [Gd(DNS-DOTA)], for the detection of the divalent copper ion(Cu2+). Luminescence lifetime measurement shows the number of inner-sphere water molecules (q) in [Gd(DNS-DOTA)] is zero. However, upon addition of Cu2+ to [Gd(DNS-DOTA)], the q value changes from 0 to 1. Consequently increase in the relaxivity of [Gd(DNS-DOTA)] was observed in the presence of Cu2+. Consistent to relaxometric study, magnetic resonance imaging (MRI) experiments display the increase in signal strength with the increase in the Cu2+ ion concentration. In addition, attenuation in the fluorescence intensity of [Gd(DNS-DOTA)] was observed on adding Cu2+ ion which substantiate that the coordination profile of [Gd(DNS-DOTA)] is alter in the presence of Cu2+ ion. High coordination preference of [Gd(DNS-DOTA)] for Cu2+ ion over other metal ion was observed under the same condition. Last but not least, confocal imaging experiment shows that the fluorescence intensity [Gd(DNS-DOTA)] suppressed on incubating with 3T3 cell pretreated with Cu2+. Therefore, [Gd(DNS-DOTA)] is a potential MR-optical imaging agent for the detection of intracellular Cu2+ ion with high specificity.
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author2 |
Wang, Yun-Ming |
author_facet |
Wang, Yun-Ming Lin, Hsin-Ping 林欣平 |
author |
Lin, Hsin-Ping 林欣平 |
spellingShingle |
Lin, Hsin-Ping 林欣平 A new gadolinium(III) complex with dansyl based copper MRI sensor |
author_sort |
Lin, Hsin-Ping |
title |
A new gadolinium(III) complex with dansyl based copper MRI sensor |
title_short |
A new gadolinium(III) complex with dansyl based copper MRI sensor |
title_full |
A new gadolinium(III) complex with dansyl based copper MRI sensor |
title_fullStr |
A new gadolinium(III) complex with dansyl based copper MRI sensor |
title_full_unstemmed |
A new gadolinium(III) complex with dansyl based copper MRI sensor |
title_sort |
new gadolinium(iii) complex with dansyl based copper mri sensor |
publishDate |
2012 |
url |
http://ndltd.ncl.edu.tw/handle/94728462100636621483 |
work_keys_str_mv |
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