A new gadolinium(III) complex with dansyl based copper MRI sensor

• Main Authors: Lin, Hsin-Ping, 林欣平
• Other Authors: Wang, Yun-Ming
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/94728462100636621483

碩士 === 國立交通大學 === 分子醫學與生物工程研究所 === 101 === In the present study, we designed and synthesized MR-optical imaging agent, [Gd(DNS-DOTA)], for the detection of the divalent copper ion(Cu2+). Luminescence lifetime measurement shows the number of inner-sphere water molecules (q) in [Gd(DNS-DOTA)] is zero. However, upon addition of Cu2+ to [Gd(DNS-DOTA)], the q value changes from 0 to 1. Consequently increase in the relaxivity of [Gd(DNS-DOTA)] was observed in the presence of Cu2+. Consistent to relaxometric study, magnetic resonance imaging (MRI) experiments display the increase in signal strength with the increase in the Cu2+ ion concentration. In addition, attenuation in the fluorescence intensity of [Gd(DNS-DOTA)] was observed on adding Cu2+ ion which substantiate that the coordination profile of [Gd(DNS-DOTA)] is alter in the presence of Cu2+ ion. High coordination preference of [Gd(DNS-DOTA)] for Cu2+ ion over other metal ion was observed under the same condition. Last but not least, confocal imaging experiment shows that the fluorescence intensity [Gd(DNS-DOTA)] suppressed on incubating with 3T3 cell pretreated with Cu2+. Therefore, [Gd(DNS-DOTA)] is a potential MR-optical imaging agent for the detection of intracellular Cu2+ ion with high specificity.