| Summary: | 碩士 === 國立中央大學 === 化學學系 === 101 === Two parts in this thesis:
Part one: annular rings with controllable sizes are successfully created on the surface of COOH-functionalized mesoporous silica (namely, XC-CAR-10, where X represents the number of carbons in the diamines used) for the adsorption of metal ions. Macrocylic effect in our annulated mesoporous silica plays a vital role in enhancing its adsorption ability for meta ions. In particular, excellent adsorption ability and selectivity towards ~300 µg/mg of Pb2+ metal ion were achieved using 3C-CAR-10. 3C-CAR-10 contains suitable geometric sizes and the cavities offer the “best-fit” for the cations for the formation of host-guest complexes.
Part two: in order to develop novel materials for protein purification, we performed annular mesoporous silica on immobilized metal affinity chromatography as a tool for protein purification. Because the annular mesoporous silica is an excellent metal ion adsorbent, e.g. Co2+ and Ni2+, the M2+-annulated materials complexes were applied for the assays of protein purification. Finally, annulated mesoporous silica showed higher binding capability of proteins with his-tags. Thus, it can open an auxiliary avenue in the development of a new resin for the protein purification process.
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