| Summary: | 碩士 === 國立中央大學 === 電機工程學系 === 101 === Bio-impedance technology extracts both physiological and pathological information from the human body through the use of biological cells, tissues, and organs. This form of testing is a non-destructive technique, which is widely in use. In our study, we used screen-printed interdigitated electrodes to detect inflammation of RAW264.7 cells in real time. We use the scanning electron microscope (SEM) to observe the cell morphology on the electrode. The relationship between impedance changed and inflammation was observed. This study also research the effect of the cells attached to the electrode at low frequencies. We will do protein quantitative analysis which will observe the relationship with impedance. In inflammation of RAW264.7 experiment for 24 hour, the inflammation groups increased normal impedance changed to 210%, while the control groups just increased normal impedance changed to 80%. In study on electrical properties of electrode surfacing with culture L929 at low frequency experiment, the results show the relationship between concentration of protein and impedance at low frequency. This study also development the impedance measurement system. The system we designed consists of two components. The first component is an AT89S51 microcontroller. The second is the integrated circuit AD5933, a bio-impedance measuring device manufactured by Analog Devices. Working together, the AT89S51 and AD5933 combine to produce a portable bio-impedance detection system. In human serum albumin experiment, the impedance measured by homemade impedance detection systsm and Im6/6ex are similar. The difference between homemade detection system and Im6/6ex are 6% or less.
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