Evaluate the cartilage induction of hyperbaric oxygen/air on stem cell-based tissue engineering in vivo

• Main Authors: Fan Gang-Yi, 范綱毅
• Other Authors: Liou Nien-Hsien
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/48040133611256590917

碩士 === 國防醫學院 === 生物及解剖學研究所 === 101 === Cartilage has a poor intrinsic healing response, and neither the innate healing response nor current clinical treatments can restore its function. Therefore, articular cartilage tissue engineering is a promising approach for the regeneration of damaged tissue. Because cartilage is exposed to compression forces during joint loading, many tissue engineering strategies use exogenous stimuli to enhance the biochemical or biomechanical properties of the engineered tissue. Hyperbaric oxygen (HBO) is a kind of high pressure gas commonly used in clinical. Literatures suggest that treatment of full-thickness osteochondral defects with HBO resulted in a clear improvement in cartilage repair. In this study, human adipose derived stem cells were seeded on the gelatin /polycaprolactone biocomposites to evaluate functional improvement of hyperbaric oxygen/air treated cartilage tissue engineering. The behavior test showed that after 2.5ATA oxygen/air treatment, the cell-based tissue engineered cartilage affect the articular cartilage defected rabbits exhibiting more beneficial characteristics in stepping distance and weight barring. Moreover, the positron emission tomography image also showed that grafted the tissue engineered cartilage can help the hydroxyapatite formation of lesion site. In the results of chemistry stain and immunohistochemistry stain, it demonstrated that the cartilage matrix synthesis of lesion site increased after grafting the high pressure oxygen/air treated tissue engineered cartilage. In conclusion, these results indicate that the hyperbaric oxygen and air treatment can provide a high pressure environment to help the cartilage matrix synthesis and improve the quality of cartilage tissue engineering in vivo