| Summary: | 碩士 === 國立高雄海洋科技大學 === 水產食品科學研究所 === 102 === PartⅠ.
The purpose of this study was to explore the replacement of fish meal with the probiotic fermented soybean meals as feed and to investigate the effects of different feeds on the growth of tilapia and giant seaperch (Lates calcarifer). There were two animal experiments. The tilapia feeds, comprising widely available and affordable raw ingredients, were iso-energetic and iso-carbohydrated, but contained protein of different sources as follows: 100% of protein from fishmeal, protein from fishmeal (80 or 60%) plus from soybean meal (20 or 40%), protein from fishmeal (80 or 60%) plus from bacterium A fermented soybean meal (20 or40%), and protein from fishmeal (80 or 60%) plus from bacterium P fermented soybean meal (20 or40%). They were called control, FLA20, FLA40, FLP20, FLP40, SBM20, and SBM40 groups, respectively. The giant seaperch feeds contained protein of different sources as follows: 100% of protein from fishmeal (control), 85% of protein from fishmeal and 15% of protein from bacterium A fermented soybean meal (FLA15), 70% of protein from fishmeal and 30% of protein from bacterium A fermented soybean meal (FLA30), and 55% of protein from fishmeal and 45% of protein from bacterium A fermented soybean meal (FLA45). Both two experiments were conducted 60-day feed trials. The survival rate, growth performance (weight gain, percentages weight gain, specific growth rate), feed digestion (feed conversion rate, feed efficiency) and body composition of two fish groups were investigated. The results indicate that SBM20 group exhibited the highest weight gain and visceral somatic index among tilapia groups. Control diet demonstrated the best growth performance. FLP20 demonstrated the best feed digestion and utilization with the experimental diets. In the seaperch experiment, the control group exhibited the highest weight gain, visceral somatic index and hepatosomatic index among seaperch group. FLA15 and FLA30 group exhibited the best growth performance and the best feed digestion and utilization among all group, respectively. In addition, the amount of essential amino acids in amino acid composition of FLA30 is the highest among all groups.
PartⅡ.
In this experiment, roe hydrolysate (RH) was produced from roes treated with enzymatic hydrolysis and defatting. To screen peptides with tyrosinase inhibitory activity, RH was fractionated and purified by anion exchange chromatography and then gel filtration (G-25) chromatography. A fraction with 78.20% tyrosinase inhibitory activity was obtained. Finally C18 HPLC column was used for further separation. A tyrosinase inhibitory peptide was purified and its amino acid sequence. Tyrosinase inhibitory peptides can be used in the development of skin care products.
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