| Summary: | 碩士 === 國立高雄海洋科技大學 === 水產食品科學研究所 === 103 === There are several problems associated with raising carnivorous fish in these high-density fish farm pens, one of the biggest being pollution, which results from uneaten food and fecal matter. In high-density fish culture that intermittent food deprivation can be used as a technology strategy. The present study cloned partial genes of peroxisome proliferator activated receptors α (PPARα) of the giant grouper (Epinephelus lanceolatus) and investigated their expressions and lipid-metabolism related growth parameters in the intermittent food deprivation condition. The giant grouper had ad libitum (control group) access to food or were intermittent food deprivation (IFD) model for 2, 4, 6 days again feeding 3 days in a 4-week growth trial. The result showed that was cloned PPARα gene from giant grouper’s cDNA contained 469 bp, the open reading frames encode 155 amino acids. The real-time PCR (qPCR) analyses showed that expression of PPARα mRNA in liver and adipose tissues. The growth parameters were not significantly different in the different intermittent food deprivation model. The IFD 2d group and IFD 6d group that liver PPARα mRNA expression were significantly up regulation expression. In the adipose tissues, the IFD 4d group and IFD 6d group that PPARα mRNA expression were significantly down regulation expression. In the intermittent fasting mode, the Concentrations of plasma triglycerides (TG) and total cholesterol (TC) were gradually enhancing. In summary, the ntermittent food deprivation 2 days and 4 days can be used as a technology strategy that reduced feed cost and farm pens pollution.
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