Determination of Flavonoids in biological sample using iron oxide nanoparticles combine with capillary eletrophoresis

• Main Authors: Chia-Wei Chang, 張家瑋
• Other Authors: Ming-Mu Hsieh
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/52485492830666767241

碩士 === 國立高雄師範大學 === 化學系 === 101 === ABSTRACT In this study, unmodified magnetic Fe3O4 nanoparticles(NPs) were used for selective extraction and enrichment of flavonoids namely- catechin (C), epicatechin (EC), rutin (R), and quercitrin (Q), prior to their analysis using capillary electrophoresis with UV detection. Coordination of Fe3+ ions present on the surface of Fe3O4 to catechol with special interactions enables the magnetic NPs to capture the catechol moiety of the flavonoids from an aqueous solution. Under the optimum extraction conditions, the limit of detections at signal-to-noise of 3:1 for C, EC, R and Q were found to be 0.82, 0.76, 0.02 and 0.02 μM, respectively. enrichment factor of up to 156.04 - 451.79. Notably, the C18 solid-phase extraction (SPE) combined with Fe3O4 NPs Magnetic solid phase extraction and the proposed method was successfully applied for the determination of C, EC, and Q in the human urine. The accuracy of this method was evaluated by the recovery of analytes in the spiked sample. Good recoveries (99.36-102.66%) with low relative standard deviations (2.70 to 3.89%) were achieved. Some advantages of the proposed methods have high sensitivity, high precision, and high accuracy.