| Summary: | 碩士 === 國立屏東教育大學 === 化學生物系碩士班 === 101 === Human progesterone receptor membrane protein (Progesterone Receptor Membrane Component 1 , PGRCM 1) the study was to investigate the protein; Recent studies have pointed out, PGRMC1 Council and heme binding, but its specific physiological role has not yet been identified in the organism. In addition, although the name PGRCM 1 literature that progesterone receptor membrane protein for the human, but in fact do not directly bind progesterone, but with an unknown protein steroid / drug binding protein (S/D-BP) for Progesterone binding message passing and metabolism; expressed concern for the results of this literature. Therefore, in order to further understand the PGRCM 1 and progesterone combined with the actual situation, this study lab colleagues previously applied transgenic technology in Escherichia coli (E.coli) in a large number of performance PGRMC1; and were added to the culture of Heme precursor ALA (5-Aminolevul Acid Hydrochloride) in the culture medium; and in developing purification after adding hemin (Hemin Chloride) reconstruction, obtained by these two methods PGRMC1 iron containing metal center and comparison. We further use of UV - visible spectroscopy (UV-Vis) confirm PGRMC1 with metal centers of iron oxidation state (Fe3 +), and the use of quartz crystal microbalance QCM (Quartz Crystal Microbala) analysis of the binding constant between progesterone; also Discussion between PGRMC1 and binding of Heme.
The results of this thesis show, PGRMC1 does not directly bind with Progesterone, but PGRMC1 active center Heme reconstruction may impact combined with Progesterone situation; while PGRMC1 and Heme Kd value can be achieved between 10-8.
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