Increasing the Content of Polymethoxy Flavones (PMFs) for Citrus depressa Hayata by Optimizing the Heat Treatment Process

• Main Authors: Li-Ping Chao, 趙麗萍
• Other Authors: Ho-Hsien Chen
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/85423604260194874473

碩士 === 國立屏東科技大學 === 食品科學系所 === 101 === Hirami lemon, Citrus depressa Hayata, belongs to the Rutaceae family, which is widely distributed over Okinawa area of Japan and Taiwan. The peel of Citrus fruit contains abundant flavonoids compounds, which can develop into health care products to enhance its value added. The previous study show that citrus fruits are rich in special groups of flavonoids called polymethoxyflavones (PMFs). PMFs are demonstrated that it belongs to flavones family and it is help to health. The peel of Hirami lemon has many kinds of PMFs. There are two major compounds, nobiletin and tangeretin, that they have been confirmed to have pharmacological activities such as anti-inflammatory, anti-atherogenic properties, anti-carcinogenic, and anti-tumor activities. However, few literatures mentioned that the impact of thermal treatments to PMFs contents in processing process. This study investigates that the impact of different thermal treatment conditions for nobiletin and tangeretin, and using Taguchi method to find optimal operation conditions. The orthogonal array experiment (L9) was used to minize the process of superheated steam, water activity and hot-air temperature. The results show that contents of nobiletin and tangeretin of freeze dried Hirami lemon are identified by HPLC and LC/MS. There are 8.56 mg/g and 4.27 mg/g respecting. Optimum conditions of thermal treatments for squeeze residues are obtained by Taguchi method are as follows: superheated steam treatment 1 min, and then 90°C hot-air drying to below 0.5 of water activity. Nobiletin will be enhanced to 30.6% with the content 11.18 mg/g. Likewise; tangeretin will be enhanced to 41.9% with the content 6.05 mg/g under the optimum conditions.