Detection of Canine Distemper Virus and Phylogenetic Analysis by Molecular Techniques

• Main Authors: Ying-Chin Chou, 周應欽
• Other Authors: Ming-Hui Liao
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/63438861883384860643

碩士 === 國立屏東科技大學 === 獸醫學系所 === 101 === Canine distemper virus (CDV) is an important disease and has a high prevalence for a long time. All ages are susceptible to infections, particularly in 3-6 months of age puppy. CDV is a high incidence, high prevalence and lethal disease, it causes systemic infection and central nervous system symptoms in affected dogs. CDV caused multisystem disorders and difficult to diagnose in clinical. This problem can be solved by molecular technique for disease diagnosis. The aim of this study was survey prevalence of suspected CDV infection by use reverse transcriptase polymerase chain reaction (RT-PCR), and insulated isothermal polymerase chain reaction (iiPCR), and established the phylogenetic relationship of wild-type CDV between different countries. The sensitivity of RT-PCR is 100 pg/L, the specificity was also established. The positive rate of RT-PCR and iiPCR were 42%, 41%, respectively. Although iiPCR is a time-saving technique, the reaction product can not subject to sequencing. Comparing the nucleic acid sequences obtained by RT-PCR with published strains in the GenBank. Seven of ten viral strain from positive specimen showed 99.3-94.9% identity with other country, but the other three wild-type strains had low identity. Further investigation is required for understanding the phylogenetic difference.