| Summary: | 碩士 === 國立清華大學 === 生物資訊與結構生物研究所 === 101 === Synthetic biology aims both to employ standard interchangeable components to mimic biological behavior, and to construct novel artificial biological systems with prescribed functions. In this study, the GA-based searching method was used to efficiently select an adequate set of promoter-RBS components from the well-characterized promoter-RBS libraries, and robust externally tunable synthetic biological filter devices with a specified input/output (I/O) response were built in Escherichia coli. The thresholds of these gene circuits could be tuned by adjusting the concentration of the control inducer.
The green fluorescent protein (GFP) was employed as the reporter protein in the gene circuit, of which expression levels were measured using an ELISA reader. The gene expression patterns of these biological filters exhibited low-pass and high-pass like characteristics respectively in response to an input chemical gradient in vivo. In the future, different promoter-RBS components with different kinetic activities may be selected to precisely tune the threshold of the I/O response of filters, thereby making the experimental results more closely fit the model prediction. The biological filters may provide a wide variety of applications in pattern formation, biosensor, and cancer therapy.
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