Multiparametric characterization of the folding mechanism of a pseudo-knotted protein from Helicobacter pylori
碩士 === 國立清華大學 === 生物資訊與結構生物研究所 === 101 === Abstract To date, about 700 knotted proteins have been identified in the protein data bank (PDB). How and why these protein adopt knotted backbone topologies remain elusive. HP0242 is a hypothetical protein from Helicolbacter pylori which is homodimeric and...
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ndltd-TW-101NTHU51121492015-10-13T22:29:58Z http://ndltd.ncl.edu.tw/handle/13944774621385807136 Multiparametric characterization of the folding mechanism of a pseudo-knotted protein from Helicobacter pylori 多元物理參數分析對於來自於胃幽門桿菌之偽紐結蛋白折疊機制研究以及探討 Wang. Liang Wei 王亮崴 碩士 國立清華大學 生物資訊與結構生物研究所 101 Abstract To date, about 700 knotted proteins have been identified in the protein data bank (PDB). How and why these protein adopt knotted backbone topologies remain elusive. HP0242 is a hypothetical protein from Helicolbacter pylori which is homodimeric and contains a novel interwined topology (a pseudo trefoil 31 knot); it will be properly knotted when two monomers are concatenated. HP0242 is an ideal model system for studying knotted protein folding. While far-UV circular dichroism (CD) spectroscopy with both in-house and synchrotron radiation light sources identifies the existence of a highly populated folding intermediate during chemical denaturation, intrinsic fluorescence spectroscopy only probes the native (N) to intermediate (I) state transition because HP0242 only has one tryptophan residue and it is likely that the local structure around the tryptophan side chain becomes fully solvent exposed while a significant part of the structure remains folded in the intermediate state. We therefore design two Phe-to-Trp mutants to monitor folding events at the other sites of HP0242. Together with small angle X-ray scattering (SAXS) and dynamic light scattering (DLS), which report on the changes of radius of gyration (Rg) and radius of hydration (Rh), respectively, as a function of denaturant concentration, we have established the sequential folding processes of HP0242 during chemical denaturation. 6 In light the of the emerging interests in employing theoretical modeling approaches to delineate the folding pathways of knotted proteins, our results provide key experimental evidence of sequential folding events of HP0242 which can be validated in the theoretical models. Hsu, Ban-Dar Hsu, Shang-Te Danny 徐邦達 徐尚德 2013 學位論文 ; thesis 69 en_US |
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碩士 === 國立清華大學 === 生物資訊與結構生物研究所 === 101 === Abstract
To date, about 700 knotted proteins have been identified in the protein data bank
(PDB). How and why these protein adopt knotted backbone topologies remain elusive.
HP0242 is a hypothetical protein from Helicolbacter pylori which is homodimeric
and contains a novel interwined topology (a pseudo trefoil 31 knot); it will be properly
knotted when two monomers are concatenated. HP0242 is an ideal model system for
studying knotted protein folding. While far-UV circular dichroism (CD) spectroscopy
with both in-house and synchrotron radiation light sources identifies the existence of a
highly populated folding intermediate during chemical denaturation, intrinsic
fluorescence spectroscopy only probes the native (N) to intermediate (I) state
transition because HP0242 only has one tryptophan residue and it is likely that the
local structure around the tryptophan side chain becomes fully solvent exposed while
a significant part of the structure remains folded in the intermediate state. We
therefore design two Phe-to-Trp mutants to monitor folding events at the other sites of
HP0242. Together with small angle X-ray scattering (SAXS) and dynamic light
scattering (DLS), which report on the changes of radius of gyration (Rg) and radius of
hydration (Rh), respectively, as a function of denaturant concentration, we have
established the sequential folding processes of HP0242 during chemical denaturation.
6
In light the of the emerging interests in employing theoretical modeling approaches to
delineate the folding pathways of knotted proteins, our results provide key
experimental evidence of sequential folding events of HP0242 which can be validated
in the theoretical models.
|
author2 |
Hsu, Ban-Dar |
author_facet |
Hsu, Ban-Dar Wang. Liang Wei 王亮崴 |
author |
Wang. Liang Wei 王亮崴 |
spellingShingle |
Wang. Liang Wei 王亮崴 Multiparametric characterization of the folding mechanism of a pseudo-knotted protein from Helicobacter pylori |
author_sort |
Wang. Liang Wei |
title |
Multiparametric characterization of the folding mechanism of a pseudo-knotted protein from Helicobacter pylori |
title_short |
Multiparametric characterization of the folding mechanism of a pseudo-knotted protein from Helicobacter pylori |
title_full |
Multiparametric characterization of the folding mechanism of a pseudo-knotted protein from Helicobacter pylori |
title_fullStr |
Multiparametric characterization of the folding mechanism of a pseudo-knotted protein from Helicobacter pylori |
title_full_unstemmed |
Multiparametric characterization of the folding mechanism of a pseudo-knotted protein from Helicobacter pylori |
title_sort |
multiparametric characterization of the folding mechanism of a pseudo-knotted protein from helicobacter pylori |
publishDate |
2013 |
url |
http://ndltd.ncl.edu.tw/handle/13944774621385807136 |
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