| Summary: | 碩士 === 國立臺南大學 === 生物科技學系碩士班 === 101 === Stanniocalcin (STC) formerly called hypocalcin or teleocalcin, is a
50-kDa disulfide-linked homodimeric glycoprotein that was originally
identified in fish and secreted from the corpuscles of Stannius (CS),
which are attached to the kidneys. Calcium is essential element for all
animals. It is important for bone structure, and it serves a variety of
intracellular and extracellular roles. For terrestrial vertebrates, food is the
main source of Ca2+, while aquatic vertebrates obtain Ca2+from water .
Ca2+ absorption occurs in epithelia, including the kidney, intestine, and
gill.
In this study, the partial mRNA sequence of STC-1 and ECaC was
confirmed by gene cloning. The specific primer was designed by the
sequence of database. Using PCR , STC-1 and ECaC gene expression
was amplified in every stage during embryo development. This result
prove STC-1 and ECaC gene express in early stage. The effect of
environment in different calcium concentration on calcium content during
embryo development and the relation between STC-1 and ECaC will be
the purpose.
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