A microwave-assisted fluorescent labeling method for the separation and detection of amphetamine-like designer drugs by capillary electrophoresis.

• Main Authors: Kuan-Fu Chen, 陳冠甫
• Other Authors: Cheng-Huang Lin
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/53663304968387800567

碩士 === 國立臺灣師範大學 === 化學系 === 101 === A microwave-assisted fluorescence labeling method for use in CE-LIF （capillary electrophoresis-laser induced fluorescence） is described. Six amphetamine-like designer drugs, namely, o-, m-, p-chloro- and o-, m-, p-fluoro-amphetamine derivatives, and five 2C-series of phenethylamine designer drugs, including 2,5-dimethoxy-4-ethylthio-phenethylamine （2C-T-2）, 2,5-dimethoxy-4-（n）-propylthiophenethylamine（2C-T-7）, 4-chloro-2,5-dimethoxy-phenethylamine（2C-C）, 4-bromo-2,5-dimethoxy- phenethylamine（2C-B）, 2,5-dimethoxy-4-iodo-phenethylamine（2C-I） derivatives, were synthesized and used as model compounds. FITC （fluoresceinisothiocyanate isomer I） and a blue-laser were used as the fluorescent labeling reagent and excitation source, respectively. When a microwave oven was used, the reaction was complete within ~ 5 min,while the classical method required at least 20 h（usually, an overnight reaction）