| Summary: | 碩士 === 國立臺灣海洋大學 === 水產養殖學系 === 101 === Surfactin is an amphipathic cyclic lipopeptide molecule derived from aquatic
probiotics, Bacillus subtilis, secondary metabolites. This simple molecule also looked
very promising as an anti-bacteria, anti-virus, anti-tumor, anti-coagulant and
transdermal penetration enhancer. The objectives of present study are used surfactin
produced by Bacillus subtilis derived from fishpond in Thailand, 1） to know the
surfactin anti-aging function and the effects of mouse embryo fibroblast and human
skin fibroblast aging mechanism, 2） to new purification for surfactin products from
semi-solid state fermentation method, 3）to study the function of transdermal
penetration enhancer and safety test. The present results showed that surfactin can
up-regulated the expression of sirtuin I and sirtuin III effectively and proliferation of
fibroblast by treatment with 25 μM to 100 μM surfactin with culture medium.
Surfactin also can increased cell survival rate and repair ability after oxidative stress
and UV-A damage, and induced collagen and inhibited in MMP-9 of fibroblast. Using
surfactin as a transdermal penetration enhancer, the results for permeability assay
demonstrated that 50 ppm purified surfactin was capable in enhancing the
permeability of gold-nanoparticle. The result for percutaneous absorption indicated
that 9.7 ％ subcutaneous penetration with treated surfactin to 2 hours. Surfactin
safety test showed that the degree of cytotoxicity was 2, having weak allergenic
potential and no skin irritation. These results indicated that surfactin could induce
anti-aging function and extracellular matrix by efficacious stimulation on the
fibroblast. Surfactin consists of a heptapeptide headgroup with the sequence
Glu-Leu-D-Leu-Val-Asp-D-Leu-Leu closed to a lactone ring by a C14 β-hydroxy fatty.
Surfactin also enhancing the penetration of Au-NPs. Surfactin serve as anti-aging
activator and transdermal penetration enhancer, and it might be applied in anti-aging
cosmeceutical and pharmaceutical industry.
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