| Summary: | 碩士 === 國立臺灣大學 === 化學研究所 === 101 === Thermophilic fungi are a special group of fungi. In 1964, Cooney and Ewerson defined that this group of fungi have a minimum growth temperature higher than 20℃ and could grow at temperature above 50℃. Thermophilic fungi are the only group of eukaryotes that could live at above 45℃. However, our understanding of this unique organism is still little compared to thermophilic bacteria or archaea. Because of their unique growth condition, they have huge potential for useful drug or enzyme discovery. In this experiment, we chose strain Myceliophthora sp. T31 from the fungi species Myceliophthora thermophila as our studying material, trying to figure out if there are any bioactive compounds that can be found from the secondary metabolites of this fungus.
This species, Myceliophthora thermophila , has an optimal growth temperature at 35-48℃. We found out that the strain Myceliophthora sp. T31 grew much slower at 55℃. In addition, it started to produce a lot of dark secondary metabolites under high temperature. Preliminary bioactivity tests showed that the ethyl acetate extract had anti-cancer activity and the methanol extract had anti-inflammatory activity. The special growth pattern of this fungus and the bioactivity potential of its secondary metabolites had drawn our interest. As a result, we decided to scale up this unique strain of Myceliophthora thermophila at 55℃, and using a series of chemical tools to identify the compounds and their bioactivity in the fungus secondary metabolites extracted by both ethyl acetate and methanol.
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