Effect of protein adsorption on electro-osmotic flow in nano-slits

• Main Authors: Yen-Jun Huang, 黃彥鈞
• Other Authors: Si Wei Chu
• Format: Others
• Language: en_US
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/79396070725371183993

碩士 === 國立臺灣大學 === 應用物理所 === 101 === Electrokinetically-driven nanofluidics has been widely used in micro-total-analysis system (μTAS), especially for proteomic analysis. However, many details in how proteins were absorbed onto the surface and perturbed the electro-osmotic flow (EOF) are still elusive. In this study, we investigated the reliability of current-monitoring method by reproducing the results under different conditions, and used this method to measure electro-osmotic mobility under different concentration of bovine serum albumin (BSA) in nano-slits (channel height ~ 500nm). We observed that EOF mobility would be reduced as BSA concentration increases, and reach a saturation level at high BSA concentration. By fitting with exponential decay from random-sequential-adsorption model suggested by Adamczyk et al1,2, two relevant parameters were found: characteristic perturbing concentration is 1.4±0.3 mg/ml and mobility at high concentration is 1.3±0.1 um*cm/V s.