Analyze and identify the anti-tumor compounds fromChinese olive extracts and its physiological activity

• Main Authors: Yu-Te Yeh, 葉昱德
• Other Authors: 謝淑貞
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/86432803070265296501

碩士 === 國立臺灣大學 === 食品科技研究所 === 101 === Chinese olive is a traditional medicine material, exhibiting beneficial functions in anti-bacterium, anti-virus, anti-inflammation and detoxification. Previous studies have displayed Chinese olive a phenolic compounds rich resource for providing antioxidative activity. However, the role of Chinese olive in anti-cancer remains unknown. Therefore, our aim is to search for compounds with anti-proliferation effect in Chinese olive. In this study, we used WST-1 assay to examine the anti-proliferation function of compounds within Chinese olive. Firstly, we obtained the ethyl acetate fraction from Chinese olive methanolic extract and named it as OM-EtOAc. We then found that the subfraction of OM-EtOAc-C and OM-EtOAc-D from OM-EtOAc through Diaion HP-20 column chromatography exhibited better antitumor effect than the other subfractions. These two subfractions were then precipitated by 90% Hexane / Ethyl acetate. The supernatant of OM-EtOAc-C revealed superior anti-tumor activity to the precipitate, whereas both the supernatant and precipitate of OM-EtOAc-D displayed comparable effect. We then used HPLC and TLC spot to titrate appropriate separation conditions for separating the supernatants of OM-EtOAc-C and OM-EtOAc-D (OM-EtOAc-C2 and OM-EtOAc-D2), followed by chromatography on silica gel. The OM-EtOAc-C2b revealed better effect thanOM-EtOAc-C2, however, no fraction exhibited higer anti-tumor activity than OM-EtOAc-D2. Finally, we choose OM-EtOAc-C2b to identify functionalcompounds using LC-SPE-NMR and LC-MS, and 12 compounds were identified.Among the compounds identified, three remained to be further analyzed to confirm the chemical structure. In animal experiments, We also implanted mouse CT26 colon cancer cells to BALB/c mice and allowed them to form tumors. The tumor-bearing mice were treated with either high dose of OM-EtOAc (300mg/kg bw/day) or lowdose of (150mg/kg bw/day) OM-EtOAc. Compared with control mice, OM-EtOActreated mice beared significantly smaller tumor after four-week treatment. In conclusion, Chinese olive has anti-tumor proliferative effect, and the functional compounds includee gallic acid, methyl gallate, ellagic acid, esculetin, scopoletin.