| Summary: | 博士 === 國立臺灣大學 === 農業化學研究所 === 101 === We studied the effects of nonylphenol (NP) and octylphenol (Giannopolitis and Ries) on physical features and proteome of Arabidopsis (Arabidopsis thaliana) during growth. Biomass and root length were decreased after 10 days of NP treatment. Levels of chlorophyll decreased but proline increased in leaves. Malondialdehyde (MDA), is oxidative stress indicators. The results indicated that NP and OP caused oxidative stress, and the activities of ascorbate peroxidase, catalase, CuZnSOD and MnSOD were induced in leaves under NP stress. The observed decrease in SOD activity with 50 mg L-1 NP treatment could diminish the ability of the Arabidopsis scavenge O2- radicals, thus favoring an accumulation of ROS, which could cause MDA accumulation. OP also caused similar results. Arabidopsis has the same reaction mode against NP and OP.
The proteome of leaf tissue was analyzed by 2-D gel electrophoresis and mass spectrometry. NP might adversely affect the activities of sedoheptulose 1,7-bisphosphatase, fructose-1,6-biphosphate aldolase, Rubisco and oxygen-evolving enhancer protein. The above enzymes are important enzymes in photosynthesis. OP adversely affects the activities of Anthranilate synthase component I-2, chloroplastic, Methyl-CpG-binding domain-containing protein 2 and pentatricopeptide repeat-containing protein. The above enzymes are important enzymes in chlorophyll synthesis and plant growth. The results can solve that NP and OP inhibit growth of Arabidopsis.
The results of this study indicated that NP and OP are not only endocrine disruption of animals, but also result in a considerable degree of plants damage. The residual in environment deserves continued attention and concern.
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