Preparation of Magnetic Molecularly Imprinted Polymers and Its Application in the Adsorption of Inosine

• Main Authors: Chieh-Chi Chen, 陳玠錡
• Other Authors: Nan-Wei Su
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/72486391369964643360

碩士 === 國立臺灣大學 === 農業化學研究所 === 101 === 　　Gout is one of the most common forms of arthritis .When the uric acid cannot be completely filtrated by kidney, it can form monosodium urate crystals and accumulate in the joints and form gout. The gout patients always need to select foods cautiously to avoid the foods or beverages that contain high concentration of purine. 　　In 2010, Kusunoki and Kobayashi were developed the inosine-imprinted polymers microbeads which were prepared by emulsion copolymerization of divinyl- benzene (DVB) and methacrylic acid (MAA) in the presence of inosine template and laurylbenzenesulfonic acid (LBSA) as surfactant. The resulting copolymer micro- beads were observed to have a binding behavior of Inosine and surfactant to the polymer. This demonstrated that a cooperative binding with the surfactant was cooperatively occurred in the presence of inosine.In this study, magnetic molecularly imprinted polymers (MMIPs) were successfully prepared without surfactant and composed of Fe3O4 / SiO2 / γ-MPS nanocomposite particles as the core and methacrylic acid as functional monomer for specific identification of inosine. Observed by TEM, MMIPs were uniformly-coated spherical particles with the SiO2 shell thicknesses from 25 to 30 nm and the coated shell thicknesses of MIPs from 50 to 70 nm. The average diameter of MMIPs measured by DLS was 310.1 ± 15 nm. 　　The results of adsorption experiments showed that Qmax1 was 6.01 μmol/g and KD1 was 1.58 μmol/L. Compared with Kusunoki and Kobayashi’s study, our results of Qmax1 was 1.5 times higher and the KD1 value was about 1/10, indicating that the adsorptive ability and affinity were better than previous results.