Novel Mass Spectrometry Technology Development for Virus/nanoparticle and Cell/microparticle Analysis

• Main Authors: Huan-Chang Lin, 林煥彰
• Other Authors: Richie L. C. Chen
• Format: Others
• Language: en_US
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/09653721268482662582

博士 === 國立臺灣大學 === 生物產業機電工程學研究所 === 101 === Recently, mass spectrometry was extended to detect large organic- and bio-particles in sub-nano scale. In this work, different novel technologies have been developed to aim at the detection of even larger organic- and bio-particles, such as cells/microparticles and virus/nanoparticles. The sizes ranging from nano- to micro-scale are very difficult to detect by conventional mass spectrometry. In chapter I, I give the brief introduction on the technology development. Some future perspectives on the applications are also included. In chapter II, laser induced acoustic desorption (LIAD) to desorb cells and microparticles inside a quadrupole ion trap is presented. Measurements of the masses of mammalian and poultry erythrocytes, organic microparticle and cells were achieved by the combination of LIAD frequency scanning ion trap and charge detection into one facility. The mass distributions of these particles were also determined. For virus and nanoparticles, the number of charges on each particle is too low to be accurately determined by the current charge detector. The detection of virions/nanoparticles directly by a charge amplification detector is also not feasible due to the low velocities of these nanoparticles. A novel approach was developed based on the simultaneous measurement of different sizes and different number of charges of each nanoparticle to derive the mass of nanoparticles. The details are presented in chapter III. Due to the aforementioned achievements, mass spectrometry can now be used to detect the mass region from atom to cell.