Sub-Micron Electrically Cell Patterning Technology by Surface Potential

• Main Authors: Yi-Lun Wang, 王翊倫
• Other Authors: 林致廷
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/37150829130890069199

碩士 === 國立臺灣大學 === 電子工程學研究所 === 101 === As the medical technology and micro-electromechanical systems become mature, the development of cell chips are also more and more important. It has become a hot research topic for decades to try to achieve useful and efficient cell chips by taking advantage of micro-electromechanical technology. Needless to say, to the successful development of cell chips, it is crucial how we effectively and accurately pattern biomolecules and cells. The developed patterning technologies, such as microcontact printing, photolithography, and dielectrophoretic patterning, however, cannot achieve goals of high efficiency, high patterning resolution and no damage to biomolecules. In order to solve this problem, here we have applied the technique of electrowetting, which can change the surface hydrophobicity/hydrophilicity by applying voltage. The hydrophobicity/hydrophilicity is one of the most important non-covalent forces for biomolecules. Thus, through the regulation of applied voltage, surface properties can be controlled, and therefore the attachment of biomolecules and cells can further be precisely manipulated. In this research, we prove that cell patterns can be confined in the regions of electrode patterns, and patterning resolution can be down to sub-micrometer scale. Besides, this technique can avoid unnecessary generation of electrolysis and heat, and the possible negative influence of the electric field or mechanical stress can also be excluded. Based on previous results, it is certain that this technique can provide a new direction for biomolecular and cell patterning techniques and also benefit the development of biochips and cell chips.