Aquaculture Development of different generation of Thalloid Sporophyte and Gametophyte of Grateloupia taiwanensis

• Main Authors: Shih-Hsin Tai, 戴世昕
• Other Authors: 周宏農
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/85119990204169529472

碩士 === 國立臺灣大學 === 漁業科學研究所 === 101 === Grateloupia is a commercial macroalgal which belonging to the phyla of Rhodophyta under the order of Halymeniales. Field collection from the intertidal zone of Taiwan North-eastern coast, Grateloupia has been widely consumed as a delicacy by the local people. Wild Grateloupia was hand-picked by snorkelers in an inefficient way. Thus, the production of Grateloupia was limited and unable to meet the demand from the market. Despite this fact, very few researches on the cultivation of Grateloupia were done on the production of Grateloupia at commercial scale. In this study, the propagation of Grateloupia taiwanensis nodulous filament for germ stock and seedling development was investigated. The nodulous filaments were prepared from germinating carporspores and tetraspores separately. Discoid crust from germinating spores were detached from the substrat, and maintained in enriched medium. Subsequently, various conditions to scale-up the production of nodulus filament cultures were determined. The nodulus filaments were blended into small fragments which were capable in forming new discoid crusts under appropriate conditions. At 20℃, under high illumination of light (7000 lux), young shoots formed from the crusts. The young shoots were transferred to outdoor environment for further growth under appropriate conditions. It was also observed that diploid nodulus filaments germinated from carpospores grew better at higher temperature compared to the haploid nodulous filaments, which were germinated from tetraspores. Most of the discoid crusts formed from diploid nodulous filaments and carpospores were smaller in size, and were easily detached from substrate. Therefore, we suggest that nodulus filaments from detached diploid discoid crusts is an ideal source of seedlings which can be cultivated in suspension. During its growing season, the length of thalli developed from the diploid nodulus filaments reached about 10-20 cm. However, the morphology of the thallus is significantly different from those collected from the field. We suggest that the indoor cultivation of seedling sources to be improved at larger scale. The life cycle of Grateloupia may also can be determined artificially using this technique.