EGFR-Targeting Peptide Conjugated pH-Sensitive Micelles as a Potential Drug Carrier for Photodynamic Therapy

• Main Authors: Wen-Yu Chu, 竺玟妤
• Other Authors: 謝銘鈞
• Format: Others
• Language: en_US
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/40851831275188613430

碩士 === 國立臺灣大學 === 醫學工程學研究所 === 101 === Multifunctional theranostics have recently been intensively explored to optimize the efficacy and safety. Herein , we report multifunctional mixed micelle that constructed from graft copolymer PEGMA-co-DPA and diblock copolymer mPEG-b-PCL as the carrier of hydrophobic photosensitizer, chlorin e6 (Ce6) for simultaneous photodynamic imaging and therapy. The functional inner core of PEGMA-co-DPA exhibited pH stimulate to accelerate drug release under slightly acidic microenvironments of tumors and the outer shell of mixed micelles with epidermal growth factor receptor (EGFR) – targeting GE11 peptides for active targeting of EGFR- overexpressing cancer cells. The results demonstrate that GE11-conjugated chlorin e6 – loaded mixed micelles with particle size around 100 nm and the mixed micelles had well defined core shell structure which was evaluated by TEM. From the in vitro cellular uptake studies, GE11-conjugated mixed micelles could effectively release chlorin e6 more than non- targeted mixed micelles, thereby providing a similar cytotoxic effect on the viability of HCT116 cancer cells (EGFR high expression) . In vivo study revealed that specific targeting of GE11-conjugated mixed micelles exhibited cancer targeting and efficiency expression on tumor growth, indicating that GE11-conjugated chlorin e6 – loaded mixed micelles could be successfully applied to in vivo photodynamic tumor imaging and therapy simultaneously.