| Summary: | 碩士 === 國立臺灣大學 === 口腔生物科學研究所 === 101 === Previous studies from our laboratory have demonstrated that nanodiamonds (NDs) can promote delivery of siRNA into cancer cells to enhance target gene suppression. In order to develop ND-mediated delivery into clinically applicable gene therapy for cancer, it is necessary to verify its silencing efficiency in vivo. In this study, we use tumor xenograft model to analyze the delivery efficiency of ND-carried siRNA complexes, by comparing the kinetics of target gene suppression level and its effect on tumor growth. Our results indicate that NDs can greatly promote the delivery of siRNA efficiency in vivo. On the other hand, despites of the high bio-inertness of ND, it still remains to be tested whether NDs display good in vivo biocompatibility under invasive administration scheme. Toward this end, we examined the physiological and pathological responses of mice after administration of ND by intravenous and intraperitoneal injection, and observed no significant abnormality in the ND treated mice. Taken these together, our results suggest that ND can be an excellent siRNA delivery vector for the treatment of cancer, and thus prompt us to develop a practical method for its tracking in vivo. We explored the high refractive index of NDs for imaging by tomographic phase microscopy, and identified intracellular NDs in 3 dimensions. Combining the good delivery efficiency, high biocompatibility and traceable characteristics, we believe that ND promises high potentials as drug vector and might bring great welfare in cancer therapy in the future.
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