| Summary: | 碩士 === 國立臺灣科技大學 === 醫學工程研究所 === 101 === With the changes in lifestyle and the advancement of medical engineering, biotechnology has become one of the world-wide progressive sunrise industries in recent years. Moreover, the awareness of preventive medicine has brought the huge commercial opportunities of biological specimen storage. Presently, long-term preservation of biological cells(umbilical cord blood, peripheral blood, adipose stem cells, oocytes, sperm) are mostly using ultra-low temperature(-196℃) storage system to maintain cells’ activity since all kinds of these biological cells will not be used immediately. The liquid nitrogen ultra-low temperature storage devices used by world-wide biological specimen storage industries can be divided into two types, traditionally manual and automatically robotic. For examples, Taylor-Wharton's K series and LABS series stand for traditional storage devices while Cryo-Cell system and BioArchive system represent automatic storage devices.
The main objective of this study is the development of the managing and monitoring system of ultra-low temperature, high-density, and single-access biological specimen storage. The positioning of this system emphasizes via combining the barrel body of traditional storage tank with the storage system developed in this study can achieve the function of the arranging density and single-access of automatic storage equipment. As for the follow-up maintenance and the price, the system developed in this study has a huge advantage compared with the automated storage equipment. The system can significantly reduce the various disadvantages of traditional storage devices.
The experimental procedure consists of several processes such as mechanism designing, sample manufacturing, computer simulation analysis and practical test of systemic components. The designing concepts of the storage system are drawn into model firstly, and then considered in a liquid nitrogen environment to know if the material produces low temperature embrittlement and can the production accuracy remains. Continuously, in the computer simulation analysis phase, the failure mode of the system is forecasted, and the conditions for both short-term and long-term simulation analysis are confirmed as well. The last process includes the production of the storage system entities and the practical test planning; the test contents includes investigating the probability of excessive deformation caused by the liquid nitrogen circumstances and testing the yield rate of specimen access and storage.
According to the results, by the design principles of this storage system, it can effectively reach these following improved functions-tight specimen storage, independent access to lower the short-term thermal effects, maintenance efficiency progressed by moving consumable parts upward, demisting bottom of the barrel to avoid icing. Furthermore, the results of finite element analysis and practical test validate each other in the excellent safety and stability of system operating.
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