| Summary: | 碩士 === 國立虎尾科技大學 === 生物科技研究所 === 101 === After anthocyanins extraction, the extracted purple rice was used as the main solid medium for Cordyceps militaris cultivation in this study. The effect of addition of soy sauce cake and defatted black sesame meal on production of bioactivity compounds (cordycepin, adenosine, cordycepic acid and polysaccharides) from C. militaris in solid-liquid cultivation for 60 days at 22oC and 70% RH (relative humidity) was investigated. The mixture ratios of the solid media of purple rice (PR) and soy sauce cake (SSC) or PR and defatted black sesame meal (DBSM) were set as 100%：0%, 75%：25%, 50%：50%, 25%：75%, 0%：100% (defined as 100%PR (or PR), 25%, 50%, 75%, 100%SSC or DBSM, respectively). The results showed that C. militaris could grow to form its fruiting body only cultivated in the solid media of 100%PR, 25%SSC and 25%DBSM (redefined as PR-SSC and PR-DBSM, respectively). The best cordycepin productivity from the fruiting body indicated its order for C. militaris cultivated in the media PR-DBSM. The yield of adenosine, cordycepic acid and polysaccharides of the fruiting body showed its order for C. militaris cultivated in the media PR-SSC. This indicated that the addition of SSC and DBSM could improve the productivities of cordycepin and adenosine from the fruiting body of C. militaris, but just only the addition of SSC could improve the yields of cordycepic acid and polysaccharides of the fruiting body. However, the formation of the fruiting body was totally inhibited when the addition of SSC or DBSM was more than 25%. The highest yields of cordycepin, adenosine and cordycepic acid from the solid media indicated that C. militaris mycelia cultivated in the media 50%DBSM, 50%SSC and 100%PR, respectively. The yields of polysaccharides from the solid media showed that there was no significant difference for C. militaris mycelia cultivated in all the solid media except for 100%DBSM with the less yield effect.
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