| Summary: | 博士 === 中國文化大學 === 體育學系運動教練碩博士班 === 101 === Proteomics is the performance of genomic complement proteins, and proteomics is the protein level on functional genomics. In this study, proteomic strategies to detect and relatively elite sprinter (sprint runner group, SR), long distance runners (long-distance runner group, LDR) and theuntrainedcontrol group (control group, CON) between serum proteins. Purposes: To identify the sprint and long distance runners marathon specific serum protein and comparison of two different types of track athletes difference between the serum proteome. Methods: Serum protein fractionations were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and then analyzed by a quantitative nano-LC-MS/MS-based proteomic profiling. The one-way analysis of variance (ANOVA) followed by Scheffe post hoc comparison was used to determine whether there is any significant difference of each protein level between three groups, the significance level (alpha value) was set at .05. Results: (1) Of the 307 identified proteins, there were 26 unique proteins in the SR group, and 18 unique proteins in the LDR group. (2) The expression levels of 7 coagulation function-associated proteins (vitronectin, serum paraoxonase/arylesterase 1, fibulin-1, complement C3, vitamin K-dependent protein, inter-alpha-trypsin inhibitor heavy chain H3 and von Willebrand factor) were significantly decreased in the group LDR compared to group SR. (3) The expression levels of 2antioxidantproteins,afamin and glutathione peroxidase 3, were also significantly decreased in the group LDR compared to group SR. (4) The expression levels of 7 immune function-related proteins (Ig gamma-3 chain C region, Ig lambda-like polypeptide 5, clusterin, complement C1s subcomponent, complement factor B, complement C4-A, complement C1q subcomponent subunit A) were significantly decreased in the group LDR compared to group SR. Conclusion: This study results clearly identified the potential serum protein markers for elite sprinters and long distance runners. The changes in regulation of coagulation, antioxidant or immune function-specific proteins may also provide further clinical applications for these two different track athletes.
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