| Summary: | 碩士 === 實踐大學 === 食品營養與保健生技學系碩士班 === 101 === Bombyx batryticatus, an oriental crude drug, has been frequently used in China for thousands of years. The silkworm larva, Bombyx mori L. is mummified often being in infected by Beauveria bassiana during the larval growth stages. In traditional Chinese medicine, B. batryticatus is widely used clinically in treating detoxification, cholesterol reducing, anticonvulsion, composure, and phlegm eliminating. Several studies indicated that the pharmacological effects of B. batryticatus are closely related with its active ingredients. Beauveria species also have been widely used as biological control agents against agricultural and forestry pests. In this study, B. bassiana strain isolated from one of three samples of B. batryticatus, and identified with nuclear ribosomal DNA (rDNA) internal transcribed spacer (ITS-5.8S-ITS2) sequences analysis. The results showed that some white B. batryticatus chinese medicine without life B. bassiana, so the isolation and identification technology can be used as quality reference of the herbal medicines B. batryticatus. The C/N ratio of cultural medium effecting on B. bassiana growth, antibacterial ability antioxidant activity extracellular and intracellular enzymes synthesis and metabolites pattern was explored. Results showed that B. bassiana could inhibit Aspergillus niger, Penicillium chrysogenum and Pseudomonas aeruginosa growth. Methanol extract of B. bassiana has good antioxidant capacity including the DPPH radical scavenging, ferrous ion chelating ability, reducing power and total antioxidant capacity. The semi-quantitative API ZYM system analysis showed that B. bassiana could produce strong activities of acid phosphatase, phosphoamidase, β-glucosidase and β-glucosaminidase in all medium. In B. bassiana fermentation products, there were two pharmacologically active metabolites, imipramine and pergolide, have been detected by high performance liquid chromatography tandem mass spectrometry (LC/MS/MS). The cultured whole broth from 200L fermentor also has been proved with good antioxidant capacity and with active compounds imipramine and pergolide. Acute toxicity test showed no adverse reactions after oral administration of Sprague-Dawley (SD) rats at single dose of 2 g/kg.
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