Study on the cultivated conditions and the anti-fatigue effect ofCordyceps militaris

• Main Authors: Huang,Yu-ting, 黃郁婷
• Other Authors: 張惠香
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/32819253989422070683

碩士 === 大仁科技大學 === 生物科技研究所 === 101 === Cordyceps militaris is a herbal medicine grew on infected insect larvae or pupae. The Cordyceps militaris contains functional ingredients including cordycepin, cordyceps acid, nucleoside and Cordyceps polysaccharide, and is an important medicine in health and medicare. The deep ocean water（DOW）, which contains rich ionic minerals and trace elements that could not be synthesized by human body and could only be supplemented by intake, and also contains trace elements Mg2+ and Ca2+ ions, is suitable for promoting yeast growth or for culturing the mycelia of Aspergillus and Antrodia camphorate. The results of this study show that the Cordyceps militaris, liquid-cultivated in deep ocean water obtained from different manufacturers, showed no significant difference in quality and content of the mycelium. In the aspects of total polysaccharide content, the Cordyceps militaris cultivated in the Taiwan Yes 100% (TY100) deep life force deep ocean water is found to be relatively higher, thus such water is chosen as clultivation liquid in the follow-up study. Concentrated DOW, in different concentrations, was evaluated for the effect of adding excess ions on Cordyceps militaris cultivation. The preliminary result indicated that the use of DOW in the original concentration can increase the total polysaccharide content significantly. We further used individual Mg2+, Ca2+, K+ ions at concentration equivalent to that in DOW of the original concentration in the follow-up experiments. The results showed that the use of deionized medium with added Mg2+, Ca2+ and K+ ions can resulted in increased cordycepin content, while Mg2+ ions can improve the total polysaccharides content. In the solid-state culture, the use of TY 100 at 50% concentrations yielded fruiting bodies with greater biomass content. Meanwhile, the use of TY in 75% concentration, with added soybean meal (SM), raised the content of cordycepin and total polysaccharide. Comparing irradiation effect under different light quality, the green light afforded the greatest bacteroid and total polysaccharide content in Cordyceps militaris cultivated in solid culture. Concentration of 10 mg / mL in the antioxidant capacity DPPH scavenging effect of free radical with Vitamin C. Comparing irradiation effect under different light quality, the green light afforded the greatest bacteroid and total polysaccharide content in Cordyceps militaris cultivated in solid culture. Another feeding of Cordyceps militaris commercially available products animal model anti-fatigue assessment methods to do testing in accordance with the Department of Health announced the health food was adopted, and mice swimming endurance exercise was performed. Cordyceps militaris-treated mice was evaluated for change in blood glucose, blood lactate, blood urea nitrogen and glycogen concentration before and after exercises, in order to investigate the efficacy of Cordyceps militaris in fatigue reduction after exercise. Fifty male adult SD rats of 10 weeks of age were studied by dividing them into five groups. Except for the control group, the positive control group and the rest o three groups were all fed with 0.5, 1 and 5 fold Cordyceps militaris products in the experiment. After 28 days of feeding, the result showed that the experimental groups exhibited longer exercise time and higher glycogen concentration than that of the control group. Moreover, blood urea nitrogen and blood lactic acid content of the experimental groups was also found to be significantly lower than that of the control group. Besides, the result suggested that the feeding of Cordyceps militaris products could reduce the degree of fatigue and improve the physiological condition of the tested rats.