Phylogenetic Analyses of Human Adenoviruses (HAdVs) In Sewages of Taiwan Area

• Main Authors: Meng-Hsin Shih, 施孟欣
• Other Authors: Hsion-Wen Kuo
• Format: Others
• Language: en_US
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/38178404210456149717

碩士 === 東海大學 === 環境科學與工程學系 === 101 === The Human adenoviruses (HAdVs) have been identified as an etiologic agent causing gastroenteritis, conjunctivitis, and upper respiratory infection diseases in susceptible individuals or localized/ immunocompromised patients. The HAdVs currently have 52 serotypes classified into seven species, from A to G. Some studies in Taiwan have detected enteroviruses in spas, drinking water, hot tubs, and swimming pools of Taiwan area. However, the distribution of HAdVs in Taiwan has not been fully understood. The foremost objective of this study was to survey geological dispersal of HAdVs in sewages of Taiwan area based on phylogenetic analysis of partial hexon genes and quantification of real-time PCR assay. The results showed that nested PCR successfully amplified HAdV hexon genes (~143 bps) from all the 16 sewage samples. Sequencing results demonstrated HAdV-F41 was the dominant serotype (~87.8% and ~99.8% for cloning and high-throughput approaches, respectively) while rests of revealed sequences (~12.2 and ~0.2% for two sequencing methods) were belonged to C2, C6, and D8 serotypes. Serotype F41 was detected in 14 sewage samples. Based on Unifrac analyses, the seasonal difference had more significant effects on HAdVs distribution in Taiwan (80.8% and 68.3% variation for cloning and high-throughput approaches, respectively) than geological variation (7.2% and 10.1%). Furthermore, Quantitative results of real-time PCR assay for all six adenovirus species (A-F) were ranged from 8.8×102 to 1.7×108 viral particles/L; and, concentrations of serotypes F41 were from 7.9×102 to 1.8×108 viral particles/L. This study demonstrated sewages in Taiwan contained significant amount of HAdVs (especially species F and C).