New Coenzyme A Fluorescence Detecting Platform : Applicable toMonitoring Acetyl Transferases

• Main Authors: Ming-Hui Wang, 王茗卉
• Other Authors: Sheng-Tung Huang
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/yx7ufg

碩士 === 國立臺北科技大學 === 化學工程研究所 === 101 === The thiols are part of endogenous antioxidant system that maintains the cells’ normal redox potential and prominent biomarkers. Previously our group had prepared and demonstrated a new long-wavelength latent florescent probe, BCC, to detect Cys and GSH. We expect to extend the application of BCC for assaying CoA and establishing platform applicable to monitor acetyl transferases. We have successful implemented the first long-wavelength latent fluorogenic substrate BCC as the off-on flourimetric indicators for detecting CoA; the fluorescence signal generated by this assay is specific. Furthermore, BCC, acetyl-CoA, and CAT or CrAT with the presence of corresponding substrate are easy assay platform for real time monitoring either CAT or CrAT enzymatic activities. In-retrospect, BCC, acetyl-CoA, and CrAT is a sensitive fluorimetric indicator for quantitatively measuring carnitine in the nanomolar ranges. This CoA platform for carnitine is expected to be applicable to measure broad ranges of important physiological analyte for the clinical diagnostic.