| Summary: | 碩士 === 國立臺北科技大學 === 有機高分子研究所 === 101 === ATP synthase is a composite membrane protein, which is a combination of two different functions of the protein, namely, Fo-ATPase and F1-ATPase. In the condition, ATP synthase will synthesis of ATP from ADP and phosphate, and pump protons to the extracellular. ATP synthase is widely studied, but mostly require addition of fluorescent, actin or other large molecules. We propose here a direct approach for measuring the reaction. In this system, the increased proton concentration can be detected using a Si-nanowire based field-effect-transistor. It provides a real-time, high sensitivity platform, which can also give additional information associated with the reaction mechanism. At present, our experimental results already show evidence of the protons pumped through the Fo-ATPase.
|
|---|
