Detection of mycobacterium tuberculosis by aptamer-based CNT biosensor

• Main Authors: Chun-Chiang Lin, 林隼強
• Other Authors: 林震
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/ky35y7

碩士 === 國立臺北科技大學 === 機電整合研究所 === 101 === Experiment substrate is a 4 inch wafer after APTS forms SAM and then it is immersed in NMP (N-methyl pyrrolidinone) solution with SWCNTs after pickling. Use lithography process and sputtering process to make the comb-shaped electrodes on the substrate. Modify SWCNTs with the sensing probes (Aptamers). EDC–NHS coupling chemistry was utilized for aptamers integration onto the SWCNTs. In this research, we used the aptamer specificity to detect the MTB in the droplets. Aptamer is an synthetic oligonucleotide which can combine with different substance (such as metal ion, molecule, protein.etc.), the selectivity, specificity and the affinity of it is the same as the other anti-body, even better than them. As the MTB combined with the aptamer, there will be a significant change in the current of the CNTFET(Carbon-Nano-Tubes Field Effect Transistor). We had used the PDMS process to produce a bio-sensor chip. We expect that the bio-sensor could make in array form, and had the ability that can detect different bacterium at the same time in the future.