Purification and characterization of chitosanase from Penicillium janthinellum D4

• Main Authors: Chun-Ching Huang, 黃淳靖
• Other Authors: San-Lang Wang
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/75397831873050328088

碩士 === 淡江大學 === 化學學系碩士班 === 101 === The chitosanase producing strain Penicillium janthinellum D4, was isolated from soil in Tay Nguyen University in Vietnam. Two chitosanases F1 F2 were purified from the culture supernatant by ammonium sulfate precipitation, DEAE-Sepharose column chromatography and Macro-prep DEAE Cartridge. The molecular mass of CHS was estimated to be 49kDa by SDS-PAGE. The optimum temperature, optimum pH, thermal stability, and pH stability of CHS F2 were 60℃, pH 7~9, 25~40℃, and pH 10, respectively. The activity of CHS F2 was inhibited by Fe2+,Cu2+,and Mn2+.