| Summary: | 碩士 === 大同大學 === 生物工程學系(所) === 101 === Vitros Anti-HIV 1+2 assay, used in this work, consisting of Vitros Anti-HIV 1+2 reagent and Vitros HIV 1+2 correction solution was derived from Vitros Immunodiagnostic System.
This assay based on the immunometric bridging method fall into two reaction steps. In the first step, antibody in the sample combined with the recombinant antigen immobilized onto the reacting device. In the second step, a horseradish peroxidase (HRP)-labeled recombinant HIV antigen coupling agent was added and it reacted with the captured HIV-1 or HIV-2 antibody (IgG or IgM). Then this antibody-HRP coupling mixture was detected using a luminal method. A reagent consisting of luminol and electron-transferring compounds was added to the reaction device. The luminol compound emitted heatless light because it was excited by the antibody-HRP coupling mixture. The electron-transferring compound (substituted acetanilide) enhanced the emission of light and this was read by the Vitros System. The amount of HIV 1+2 was proportional to that of antibody-HRP coupling mixture.
The aim of this work was to detect HIV using the Vitros Anti-HIV 1+2 kit and the efficacy of this method was evaluated. In the standard assay for regular value, the accuracy of negative reaction was very high (s/co value < 1.00). For the assay of dangerous value the accuracy of positive reaction was very high (s/co value > 1.00). There was significance in the differentiation experiment. There were interferences in the presence of ascorbic acid (< 2840 μmol/L), bilirubin (< 342 μmol/L), hemoglobin (< 2 g/L) and triglyceride (< 22.6 mmol/L). During such clinical examination, standard deviation, factorial negative, factorial positive and accuracy were to be taken into consideration.
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