MEDICINAL PLANTS WITH HIGH CONTENTS OF CHLOROGENIC ACID AS TOPICAL AGENTS

• Main Authors: Wei-Cheng Chen, 陳威丞
• Other Authors: Lee, Shiow-Ling
• Format: Others
• Language: en_US
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/72056987163754464663

博士 === 大同大學 === 生物工程學系(所) === 101 === Chlorogenic acid (CA) is a phenolic substance, widely present in herbs and vegetables such as honeysuckle, forsythia, Eucommia leaves and coffee. Previous studies have found that chlorogenic acid has captured DPPH free radicals and inhibit LDL oxidation and total antioxidant capacity (TEAC) and other antioxidants, promote wound healing and immune regulation capabilities. Lonicera japonica Thunb. (Caprifoliaceae), a widely used traditional Chinese medicinal plant, is used to treat some infectious diseases and it may have uses as a healthy food and applications in cosmetics and as an ornamental groundcover. The ethanol extract of the flowering aerial parts of L. japonica (LJEE) was investigated for its healing efficiency in a rat excision wound model. This study was undertaken to evaluate the therapeutic effects of topical chlorogenic acid on excision wounds in rats. A 1% (w/w) simple ointment containing chlorogenic acid or silver sulfadiazine was applied topically once a day on full-thickness excision wounds created on rats. The wound tissue was removed and used for estimation of various biochemical analyses and to observe histopathological changes with and without treatment. The plasma levels of tumor necrosis factor (TNF)-α was measured by ELISA. In vivo antioxidant activity was performed to understand the mechanism of wound healing potency. Immunohistochemical staining was performed to study the expression pattern of transforming growth factor (TGF)-β1 and collagen IV. The ointment formulation prepared with 1% (w/w) chlorogenic acid has potent wound healing capacity as evident from the wound contraction on 15th post surgery day, which was similar with that produced by 1% (w/w) silver sulfadiazine ointment. Increased rates of epithelialization were observed in the treated rats. It also improved cellular proliferation, increased TNF-? levels during early stages of wound healing, up-regulated TGF-β1 and elevated collagen synthesis in chlorogenic acid-treated group. The results also indicated that chlorogenic acid possesses potent antioxidant activity by increasing superoxide dismutase, catalase, glutathione and decreased lipid peroxidation. In conclusion, these results demonstrate that topical application of chlorogenic acid can accelerate the process of excision wound healing by its ability to increase collagen synthesis through up-regulation of key players such as TNF-? and TGF-β1 in different phases of wound healing and by its antioxidant potential.