| Summary: | 碩士 === 國立陽明大學 === 生醫光電研究所 === 101 === Carotenoids, natural pigments that protect cells from oxidative damages, play an important role in decreasing the risk of cardiovascular diseases (CVD) and age-related macular degeneration (AMD). Serum carotenoids are usually analyzed by high performance liquid chromatography (HPLC), which is time-consuming and requires expensive columns. Here we introduce a novel method that applies resonance Raman spectroscopy to enable us to measure (or detect) the amount of carotenoids in serum in blood collection tubes, without any pretreatment except for exsanguination and centrifugation. Serum carotenoids Raman spectra were measured with a multi-wavelength Raman system. We found a shift of carotenoids C=C peak (v1) position when the excitation wavelength was changed and hypothesized that this shift is due to the variation in resonance enhancement of different kinds of serum carotenoids at different excitation wavelengths. Via a global analysis algorithm, we can selectively measure the two main components of serum carotenoids, lycopene as first component and lutein, ß-carotene, ß-cryptoxanthin and α-carotene mixture as the second component. Proportions of amplitudes from two components, which contain the information of resonance Raman coefficients and the contribution of carotenoids species, can be further analyzed to provide quantitative measurement and to correlate with related diseases. The feasibility of utilizing resonance Raman spectroscopy in clinical applications is discussed.
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