| Summary: | 碩士 === 國立陽明大學 === 傳統醫藥研究所 === 101 === Our hypothesis is that the pharmacokinetics of single pure compound, single herbal extract and complex herbal formulation may provide different behavior of absorption, distribution, metabolism and excretion. This study compared the oral bioavailability and pharmacokinetic data between pure compound of the major active component, single herbal extract, and complex herbal formulation by determining the pharmacokinetics and bioavailability of geniposide in each group following intravenous and oral administrations in rats. A conscious and freely moving rat model was used in this experiment to avoid the stress caused by restraint or anesthesia. The pure compound of geniposide, Gardenia fruits (Chinese herbal name: Zhi-Zi), and extracts of a Gardenia herbal formulation (Chinese herbal name: Zhi-Zi-Chi-Tang), were administered at doses of 200 mg/kg, 4.69 g/kg, and 10.82 g/kg for oral administration and fed by gavages to rats, respectively. The above doses are equivalent to geniposide administration dose of 200 mg/kg. For intravenous group, geniposide was injected at dose of 20 mg/kg from jugular vein. The plasma samples were then assayed by HPLC. The pharmacokinetic parameters were calculated using non-compartmental analysis. The results show that after oral administration of geniposide, Gardenia fruits, and Gardenia herbal formulation, the bioavailability were 4.23%, 32.32%, and 27.17%, respectively. Gardenia fruits administration shows the highest pharmacokinetic value for Cmax, t1/2, and AUC of geniposide among the three groups. The results of oral bioavailability of geniposide also suggest that Gardenia fruits extract, single herb, is a more efficient way for geniposide, pure compound, absorption than traditional herbal formulation administration and direct pure compound administration. The conclusion reveals that herbal ingredient-ingredient or herb-herb interaction may affect the oral absorption of geniposide related herbal formulation.
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